Table 3 Experimental grouping for part 3
Grouping | Specific Procedures |
|---|---|
model | The culture medium was adjusted to pH 4 using 1 mM HCl, followed by treatment with a combination of 0.25 mM glycochenodeoxycholic acid, 0.1 mM taurocholic acid sodium salt, 0.3 mM glycodeoxycholic acid sodium salt, and 0.1 mM taurodeoxycholic acid sodium salt for 6 h |
OE-miR- 233 - 3p | Overexpression of miR- 223 - 3p combined with the same acid and bile salt treatment as described above |
OE-miR- 233 - 3p-NC | Overexpression of miR- 223 - 3p-NC combined with the same acid and bile salt treatment as described above |
OE-miR- 233 - 3p + OE-NLRP3 | Overexpression of miR- 223 - 3p combined with overexpression of NLRP3 under the same acid and bile salt conditions as described above |
OE-miR- 233 - 3p + OE-NLRP3-NC | Overexpression of miR- 223 - 3p concomitant with overexpression of NLRP3-NC under the same acid and bile salt conditions as described above |