Table 4 The dilution of antibodies
Antibodies | Dilution (application) |
|---|---|
NLRP3 | 1:1000 (WB) |
ASC | 1:1000 (WB) |
caspase- 1 | 1:1000 (WB) |
cleaved caspase- 1 | 1:1000 (WB) |
GSDMD | 1:1000 (WB) |
GSDMD-NT | 1:1000 (WB) |
IL- 1β | 1:1000 (WB) |
IL- 18 | 1:1000 (WB) |
CDK6 | 1:50,000 |
CDK4 | 1:50,000 |
cyclin D1 | 1:10,000 |
CDK2 | 1:1000 |
cyclin E1 | 1:1000 |
Caspase- 3 | 1:5000 |
Bcl- 2 | 1:2000 |
Bax | 1:2000 |
β-actin | 1:5000 (WB) |
Goat Anti-Rabbit IgG H&L(HRP) | 1:20,000 (WB) |
Goat Anti-Mouse IgG H&L(HRP) | 1:20,000 (WB) |