Fig. 8
Silencing HAGLR inhibits GC cells migration. Silencing HAGLR promoted GC cells apoptosis and ferroptosis. A. Flow cytometry assay evaluate the apoptosis rate in AGS and HGC-27 cells treated with shHAGLR or negative control (shCtrl) (Mean ± SD, n = 3, **p < 0.01, ***p < 0.001); B-E. The effects of HAGLR knockdown on intracellular ROS(B), MDA(C), Fe2+(D), GSH(E) (The scale bar: 50 μm, Mean ± SD, n = 3, **p < 0.01, ***p < 0.001); F-G. The effects of HAGLR knockdown on mitochondrial morphology (The scale bar in cell is 2µ, in Mitochondria is 50° nm); H-I. The effects of HAGLR knockdown on ferroptosis related proteins ACSL4 and GPX4