BMC Gastroenterology

Table 1 Oligonucleotide sequences and annealing temperature for quantitative real-time PCR

From: Effect of pemafibrate in reducing intestinal long-chain fatty acid absorption and hepatic fibrosis in metabolic dysfunction-associated steatohepatitis rats

Gene name		Sequence (5ʹ–3ʹ)	Annealing Temperature (°C)
Ppara	forward	CCTCGAACTGGATGACAGTG	59
Ppara	reverse	CCCTCCTGCAACTTCTCAAT	59
Cd36	forward	GCGACATGATTAATGGCACA	60
Cd36	reverse	TGGACCTGCAAATGTCAGAG	60
Mtp	forward	GCGAGTCTAAAACCCGAGTG	59
Mtp	reverse	CACTGTGATGTCGCTGGTTATT	59
Fabp1	forward	ACTGGGGAAAAGGTCAAGGC	59
Fabp1	reverse	CCCAGTGTCATGGTATTGGTGAT	59
Fapb2	forward	CCGAGAGATTTCTGGTAACGA	59
Fapb2	reverse	CAAGCTAGCCCTTCTGCATT	59
Fatp4	forward	ATGACTGCCTCCCCCTCTAC	59
Fatp4	reverse	AGTCATGCCGTGGAGTACG	59
Apoa-IV	forward	ACCCAGCTAAGCAACAATGC	59
Apoa-IV	reverse	AAGTTTGTCCTGGAAGAGGGTA	59
Actab	forward	CTGGCTCCTAGCACCATGA	61
Actab	reverse	TAGAGCCACCAATCCACACA	61
Tgfb	forward	CCTGGAAAGGGCTCAACAC	59
Tgfb	reverse	TGCCGTACACAGCAGTTCTT	59
Col1a1	forward	GTGGACAGGCTGGTGTGAT	59
Col1a1	reverse	GGGACACCTCGTTCTCCAG	59
Acta2	forward	GCTCCGGGCTCTGTAAGG	59
Acta2	reverse	GCCCATTCCAACCATCACT	59
Timp1	forward	TGCAACTCGGACCTGGTTAT	59
Timp1	reverse	AGCGTCGAATCCTTTGAGCA	59
Serpine1	forward	AGAGCCAATCACAAGGCACT	59
Serpine1	reverse	GAGGCAAGTGAGGGCTGA	59
Hprt1	forward	TCCTCATGGACTGATTATGGACA	60
Hprt1	reverse	TAATCCAGCAGGTCAGCAAAGA	60

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